A BLAST search was performed on human dual specificity testis-specific protein kinase 1 [NP_006276.2]. 

 

Blast allows us to take a sequence and search a large database to find related sequences. There are several BLAST programs to choose from. If one is starting with a DNA sequence then nucleotide blast is appropriate, however we began our investigation with a protein sequence making protein blast apt for our needs. We did a basic blastp, followed by a PSI BLAST and DELTA BLAST. We used the broad non-redundant proteins database and did not alter any of the default settings provided. For example, we did not limit our results to a particular organism because we wanted to investigate related sequences in a wide range of species.  Sequences with E values less than 1e-04 can be considered related with an rate of less than 0.01%. We found all of our hits to have E values were below 1e-04. Most hits presented E values of zero and our highest E value was 1e-145. Therefore, all of our hits can be considered homologous to our input protein sequence. All of the BLAST searches returned results which showed the presence of a conserved kinase domain in TESK1.