References

Introduction

[1] Dual specificity testis-specific protein kinase 2. Uniport Consortium.

[2] Johne C., Matenia D., Li X., Timm T., Balusamy K., Mandelkow E. (2008) Spred1 and TESK1—Two New Interaction Partners of the Kinase MARKK/TAO1 That Link the Microtubule and Actin Cytoskeleton. Molecular Biology of the Cell, 19, 1391-1403.

[3] King I and Heberlein U (2011) Tao kinases as coordinators of actin and microtubule dynamics in developing neurons. Communicative and Integrative Biology, 5, 554-556.

[4] LaLonde D.P., Brown M.C., Bouverat B.P., Turner C.E. (2005) Actopaxin Interacts with TESK1 to Regulate Cell Spreading on Fibronectin. The Journal of Biological Chemistry, 280, 21680-21688.

[5] Scorilas A, Yousef G.M., Jung K., Meyts E.R., Carsten S., Diamandis E.P. (2001) Identification and Characterization of a Novel Human Testis-Specific Kinase Substrate Gene Which Is Downregulated in Testicular Tumors. Biochemical and Biophysical Research Communications, 285, 400-408

[6] TESK1 testis-specific kinase 1 [Homo sapiens(human)]. (2015) National Center for Biotechnology Information.

[7] Toshima J, Tanaka T, Mizuno K. (1999) Dual specificity protein kinase activity of testis-specific protein kinase 1 and its regulation by autophosphorylation of serine-215 within the activation loop. The Journal of Biological Chemistry, 274, 12171-12176.

[8] Toshima J., Ohashi K., Okano I., Nunoue K., Kishioka M., Kuma K., Miyata T., Hirai M.(3), Baba T., Mizuno K. (1995) Identification and Characterization of a Novel Protein Kinase, TESK1, Specifically Expressed in Testicular Germ Cells. The Journal of Biological Chemistry, 270, 31331-31337.

[9] Toshima J., Toshima J.Y., Takeuchi K., Mori R., Mizuno K. (2001) Cofilin Phosphorylation and Actin Reorganization Activities of Testicular Protein Kinase 2 and Its Predominant Expression in Testicular Sertoli Cells. The Journal of Biological Chemistry, 276, 31449-31458.

[10] Toshima J.Y, Toshima J, Watanabe T., Mizuno K. (2001) Binding of 14-3-3β Regulates the Kinase Activity and Subcellular Localization of Testicular Protein Kinase 1. The Journal of Biological Chemistry, 276, 43471-43481.

[11] Toshima J, Toshima J.Y., Amano T., Yang N., Narumiya S., Mizuno K. (2001) Cofilin Phosphorylation by Protein Kinase Testicular Protein Kinase 1 and Its Role in Integrin-mediated Actin Reorganization and Focal Adhesion Formation. Molecular Biology of the Cell, 12, 1131-1145.

[12] Tsumura Y, Toshima J, Leeksma O.C., Ohashi K, Mizuno K. (2005). Sprouty-4 negatively regulates cell spreading by inhibiting the kinase activity of testicular protein kinase. Biochemical Journal, 387, 627-637.

Sequence Analysis

[1] www.genecards.org

[2] NCBI Website, http://www.ncbi.nlm.nih.gov/, Accessed: 14/03/2015

[3] Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.

[4] Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal OmegaSievers F, Wilm A, Dineen DG, Gibson TJ, Karplus K, Li W, Lopez R, McWilliam H, Remmert M, Söding J, Thompson JD, Higgins DMolecular Systems Biology 7 Article number: 539 doi:10.1038/msb.2011.75

[5] Sigrist CJA, de Castro E, Cerutti L, Cuche BA, Hulo N, Bridge A, Bougueleret L, Xenarios I. New and continuing developments at PROSITE. Nucleic Acids Res. 2012; doi: 10.1093/nar/gks1067
PubMed: 23161676

Structural Analysis

[1] UCSF Chimera--a visualization system for exploratory research and analysis. Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE. J Comput Chem.2004 Oct;25(13):1605-12.

[2] Protein structure prediction on the web: a case study using the Phyre server, Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009)

[3] NCBI Website, http://www.ncbi.nlm.nih.gov/, Accessed: 04/03/2015

[4] Kyte, J. and Doolittle, R. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105-132.

[5] The PyMOL Molecular Graphics System, Version 1.7.4 Schrödinger, LLC.

[6] Schrödinger Release 2015-1: Maestro, version 10.1, Schrödinger, LLC, New York, NY, 2015.

[7] Arnold K., Bordoli L., Kopp J., and Schwede T. (2006). The SWISS-MODEL Workspace: A web-based environment for protein structure homology modelling. Bioinformatics, 22,195-201.

[8] Wass M.N., Kelley L.A. and Sternberg M.J. (2010) 3DLigandSite: predicting ligand-binding sites using similar structures.NAR 38, W469-73.

[9] Phospho.ELM: a database of phosphorylation sites - update 2011"; Dinkel H, Chica C, Via A, Gould CM, Jensen LJ, Gibson TJ, Diella F. Nucleic Acids Res. 2010 Nov 9. PMID: 21062810

[10] http://www.ncbi.nlm.nih.gov/gene/?term=XP_004937191

Domain Analysis

[1] The Pfam protein families database: R.D. Finn, A. Bateman, J. Clements, P. Coggill, R.Y. Eberhardt, S.R. Eddy, A. Heger, K. Hetherington, L. Holm, J. Mistry, E.L.L. Sonnhammer, J. Tate, M. PuntaNucleic Acids Research (2014) Database Issue 42:D222-D230.

[2] NCBI website, AceView database entry for TESK1. Accessed 15/03/15.

http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=human&c=Gene&l=TESK1

[4] InterPro website http://www.ebi.ac.uk/interpro/protein/Q15569 Accessed 15/03/15

[5] UniProt website http://www.uniprot.org/uniprot/Q15569 Accessed 15/03/15

[6] Toshima J, Tanaka T, Mizuno K. (1999) Dual specificity protein kinase activity of testis-specific protein kinase 1 and its regulation by autophosphorylation of serine-215 within the activation loop. The Journal of Biological Chemistry, 274, 12171-12176.

[7] Marchler-Bauer A, Zheng C, Chitsaz F, Derbyshire MK, Geer LY, Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Lu S, Marchler GH, Song JS, Thanki N, Yamashita RA, Zhang D, Bryant SH. CDD: conserved domains and protein three-dimensional structure. Nucleic Acids Res. 2013 Jan 1;41(D1):D348-52. 2012 Nov 28.

Further Analysis

[1] Nucl. Acids Res. (2010) 38 (suppl 2): W214-W220.doi: 10.1093/nar/gkq537

[2] NCBI Website. http://www.ncbi.nlm.nih.gov/gene?cmd=search&term=10252. Accessed: 14/03/15

[3] NCBI Website. http://www.ncbi.nlm.nih.gov/gene?cmd=search&term=5244. Accessed: 14/03/15

[4] NCBI Website. http://www.ncbi.nlm.nih.gov/gene?cmd=search&term=55742. Accessed: 14/03/15

[5] Xiao X, Yang W. Actin-based dynamics during spermatogenesis and its significance. Journal of Zhejiang University Science B. 2007;8(7):498-506. doi:10.1631/jzus.2007.B0498.

[6] NCBI website, AceView database entry for TESK1. Accessed 15/03/15.

http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=human&c=Gene&l=TESK1

[7] GeneCards website http://www.genecards.org/cgi-bin/carddisp.pl?gene=TESK1 Accessed 15/03/15

[8] Qiagen website http://www.sabiosciences.com/chipqpcrsearch.php?species_id=0&nfactor=n&ninfo=n&ngene=n&B2=Search&src=genecard&factor=Over+200+TF&gene=TESK1 Accessed 15/03/15

[9] Nucleic Acids Res. 2011 Jan;39(Database issue):D691-7. doi: 10.1093/nar/gkq1018. Epub 2010 Nov 9

[10] Lukas Käll, Anders Krogh and Erik L. L. Sonnhammer. A Combined Transmembrane Topology and Signal Peptide Prediction Method. Journal of Molecular Biology, 338(5):1027-1036, May 2004.

[11] BioGPS website TESK1 entry. http://biogps.org/#goto=genereport&id=7016 Accessed 16/03/15.

Website Artwork

Images adapted from:

[1] http://www.microscopyu.com/staticgallery/fluorescence/ptk210.html Accessed: 04/03/15

[2] http://www.sebiology.org/cell/Cell_signalling.html. Accessed 04/03/15

[3] http://www.nature.com/nrm/journal/v14/n7/images_article/nrm3609-f3.jpg. Accessed 04/03/15

CELL2007 Molecular Exploration Project